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COUPLING WITH HYDROLYSIS OF ATP AND GTP DRIVES GLUCONEOGENESIS The net reaction for the conversion of pyruvate to glucose in gluconeogenesis is 2 Pyruvate4 ATP2 GTP2 NADH2 H 6 H2O g glucose4 ADP2 GDP6 Pi 2 NAD The net free energy change, G°, for this conversion is 37.

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